Recombinant:Article Title: Salmonella Typhimurium effector SseI regulates host peroxisomal dynamics to acquire lysosomal cholesterol.
Article Snippet: Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models HeLa cells (H. sapiens) ATCC CCL-2 LOT: 70056809 Monocytes derived macrophages (H. sapiens) Blood bank at the Transfusion Medicine - King Georges’ Medical University Salmonella Typhimurium Kind gift from Prof. Michael Hensel ATCC, 14028 C57BL6/J (M. musculus) Central Animal Facility, IISc Bangalore, India. .. Recombinant DNA pLJC5-3XMyc-EGFP-PEX26 Addgene 139059 pcDNA3 flag HA: SseI This study Vector backbone from #9021, Addgene pcDNA3 flag HA: ARF1 This study Vector backbone from #9021, Addgene pFPV-mCherry Addgene 20956 pET23A His tagged SseI This study N/A pQE60 myc::sseI This study N/A pQE60 sseI::myc This study N/A SseI, Syt7, and ARF1 (along with mutants) were synthesized commercially GenScript Antibodies Rabbit Anti-LAMP1 Cell Signalling Technology (CST) D401S Anti-FLAG CST 8146 Anti-myc CST 2276 Anti-Pex14 ABclonal A7336 Anti-ESYT1 ABclonal A15410 Anti-ABCD1 Proteintech 60153-1-Ig Anti-pex5 Proteintech 12545-1-AP Anti-ARF1 Proteintech 10790-1-AP Anti-GAPDH Invitrogen T0004 Anti-HA CiteAb 2367 Anti-SYT7 Novus Biologicals NBP2-22420 PIP2 antibody Novus Biologicals NBP2-76433 Goat anti-mouse IgG Alexa fluor 594 CiteAb A-11032 Goat anti-Rabbit IgG Alexa Fluor 488 CiteAb A-11034 Goat anti-rabbit IgG Cy5 Invitrogen A10523 Control Mouse immunoglobulin Invitrogen 500-M00-1MG Control rabbit immunoglobulin PeproTech 500-P00-500μg horseradish peroxidase CST 7074P2 Oligonucleotides and other sequence-based reagents ARF1 Santa Cruz SC-105086 Synaptotagmin VII (SYT7) Santa Cruz SC-41320 E-Syt1 Santa Cruz SC-95714 16 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on D ecem ber 25, 2024 from IP 2402:800:6343:b0f8:400f:1124:9738:8c7d. .. Reagent/Resource Reference or Source Identifier or Catalog Number NPC1 Santa Cruz SC-41588 ABCD1 Santa Cruz SC-41143 On-Target plusTM Control Pool Dharmacon DM D-001810-10-20 RT Primers PEX11B FWD CTTCAGTGCTCAGAGCCAAGCC PEX11B REV GCATGGCCAAGAAGAGAGCAA PEX14 FWD CAGAAAGATGGCGTCCTCGG PEX14 REV TCATCTGTCAGCCCTTTCTTCT PEX19 FWD CCCCCACAGACAGTGAAACC PEX19 REV GTTGAGGCCAGGAGGCATCTC ABCD1 FWD GCCCTCCCTGTGGAAATACC ABCD1 REV AGCTTCTCGAACTTCCAGCC ARF1 FWD GTTTGCTGTGAAGACGGTGTC ARF1 REV AGAGGATCGTGGTCTTCCCT GAPDH FWD TCGGAGTCAACGGATTTGGT GAPDH REV TTCCCGTTCTCAGCCTTGAC ACBD5::FWD HU CTGGAAACGCTGACTGCTTTGC
Synthesized:Article Title: Salmonella Typhimurium effector SseI regulates host peroxisomal dynamics to acquire lysosomal cholesterol.
Article Snippet: Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models HeLa cells (H. sapiens) ATCC CCL-2 LOT: 70056809 Monocytes derived macrophages (H. sapiens) Blood bank at the Transfusion Medicine - King Georges’ Medical University Salmonella Typhimurium Kind gift from Prof. Michael Hensel ATCC, 14028 C57BL6/J (M. musculus) Central Animal Facility, IISc Bangalore, India. .. Recombinant DNA pLJC5-3XMyc-EGFP-PEX26 Addgene 139059 pcDNA3 flag HA: SseI This study Vector backbone from #9021, Addgene pcDNA3 flag HA: ARF1 This study Vector backbone from #9021, Addgene pFPV-mCherry Addgene 20956 pET23A His tagged SseI This study N/A pQE60 myc::sseI This study N/A pQE60 sseI::myc This study N/A SseI, Syt7, and ARF1 (along with mutants) were synthesized commercially GenScript Antibodies Rabbit Anti-LAMP1 Cell Signalling Technology (CST) D401S Anti-FLAG CST 8146 Anti-myc CST 2276 Anti-Pex14 ABclonal A7336 Anti-ESYT1 ABclonal A15410 Anti-ABCD1 Proteintech 60153-1-Ig Anti-pex5 Proteintech 12545-1-AP Anti-ARF1 Proteintech 10790-1-AP Anti-GAPDH Invitrogen T0004 Anti-HA CiteAb 2367 Anti-SYT7 Novus Biologicals NBP2-22420 PIP2 antibody Novus Biologicals NBP2-76433 Goat anti-mouse IgG Alexa fluor 594 CiteAb A-11032 Goat anti-Rabbit IgG Alexa Fluor 488 CiteAb A-11034 Goat anti-rabbit IgG Cy5 Invitrogen A10523 Control Mouse immunoglobulin Invitrogen 500-M00-1MG Control rabbit immunoglobulin PeproTech 500-P00-500μg horseradish peroxidase CST 7074P2 Oligonucleotides and other sequence-based reagents ARF1 Santa Cruz SC-105086 Synaptotagmin VII (SYT7) Santa Cruz SC-41320 E-Syt1 Santa Cruz SC-95714 16 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on D ecem ber 25, 2024 from IP 2402:800:6343:b0f8:400f:1124:9738:8c7d. .. Reagent/Resource Reference or Source Identifier or Catalog Number NPC1 Santa Cruz SC-41588 ABCD1 Santa Cruz SC-41143 On-Target plusTM Control Pool Dharmacon DM D-001810-10-20 RT Primers PEX11B FWD CTTCAGTGCTCAGAGCCAAGCC PEX11B REV GCATGGCCAAGAAGAGAGCAA PEX14 FWD CAGAAAGATGGCGTCCTCGG PEX14 REV TCATCTGTCAGCCCTTTCTTCT PEX19 FWD CCCCCACAGACAGTGAAACC PEX19 REV GTTGAGGCCAGGAGGCATCTC ABCD1 FWD GCCCTCCCTGTGGAAATACC ABCD1 REV AGCTTCTCGAACTTCCAGCC ARF1 FWD GTTTGCTGTGAAGACGGTGTC ARF1 REV AGAGGATCGTGGTCTTCCCT GAPDH FWD TCGGAGTCAACGGATTTGGT GAPDH REV TTCCCGTTCTCAGCCTTGAC ACBD5::FWD HU CTGGAAACGCTGACTGCTTTGC
Control:Article Title: Salmonella Typhimurium effector SseI regulates host peroxisomal dynamics to acquire lysosomal cholesterol.
Article Snippet: Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models HeLa cells (H. sapiens) ATCC CCL-2 LOT: 70056809 Monocytes derived macrophages (H. sapiens) Blood bank at the Transfusion Medicine - King Georges’ Medical University Salmonella Typhimurium Kind gift from Prof. Michael Hensel ATCC, 14028 C57BL6/J (M. musculus) Central Animal Facility, IISc Bangalore, India. .. Recombinant DNA pLJC5-3XMyc-EGFP-PEX26 Addgene 139059 pcDNA3 flag HA: SseI This study Vector backbone from #9021, Addgene pcDNA3 flag HA: ARF1 This study Vector backbone from #9021, Addgene pFPV-mCherry Addgene 20956 pET23A His tagged SseI This study N/A pQE60 myc::sseI This study N/A pQE60 sseI::myc This study N/A SseI, Syt7, and ARF1 (along with mutants) were synthesized commercially GenScript Antibodies Rabbit Anti-LAMP1 Cell Signalling Technology (CST) D401S Anti-FLAG CST 8146 Anti-myc CST 2276 Anti-Pex14 ABclonal A7336 Anti-ESYT1 ABclonal A15410 Anti-ABCD1 Proteintech 60153-1-Ig Anti-pex5 Proteintech 12545-1-AP Anti-ARF1 Proteintech 10790-1-AP Anti-GAPDH Invitrogen T0004 Anti-HA CiteAb 2367 Anti-SYT7 Novus Biologicals NBP2-22420 PIP2 antibody Novus Biologicals NBP2-76433 Goat anti-mouse IgG Alexa fluor 594 CiteAb A-11032 Goat anti-Rabbit IgG Alexa Fluor 488 CiteAb A-11034 Goat anti-rabbit IgG Cy5 Invitrogen A10523 Control Mouse immunoglobulin Invitrogen 500-M00-1MG Control rabbit immunoglobulin PeproTech 500-P00-500μg horseradish peroxidase CST 7074P2 Oligonucleotides and other sequence-based reagents ARF1 Santa Cruz SC-105086 Synaptotagmin VII (SYT7) Santa Cruz SC-41320 E-Syt1 Santa Cruz SC-95714 16 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on D ecem ber 25, 2024 from IP 2402:800:6343:b0f8:400f:1124:9738:8c7d. .. Reagent/Resource Reference or Source Identifier or Catalog Number NPC1 Santa Cruz SC-41588 ABCD1 Santa Cruz SC-41143 On-Target plusTM Control Pool Dharmacon DM D-001810-10-20 RT Primers PEX11B FWD CTTCAGTGCTCAGAGCCAAGCC PEX11B REV GCATGGCCAAGAAGAGAGCAA PEX14 FWD CAGAAAGATGGCGTCCTCGG PEX14 REV TCATCTGTCAGCCCTTTCTTCT PEX19 FWD CCCCCACAGACAGTGAAACC PEX19 REV GTTGAGGCCAGGAGGCATCTC ABCD1 FWD GCCCTCCCTGTGGAAATACC ABCD1 REV AGCTTCTCGAACTTCCAGCC ARF1 FWD GTTTGCTGTGAAGACGGTGTC ARF1 REV AGAGGATCGTGGTCTTCCCT GAPDH FWD TCGGAGTCAACGGATTTGGT GAPDH REV TTCCCGTTCTCAGCCTTGAC ACBD5::FWD HU CTGGAAACGCTGACTGCTTTGC
Sequencing:Article Title: Salmonella Typhimurium effector SseI regulates host peroxisomal dynamics to acquire lysosomal cholesterol.
Article Snippet: Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models HeLa cells (H. sapiens) ATCC CCL-2 LOT: 70056809 Monocytes derived macrophages (H. sapiens) Blood bank at the Transfusion Medicine - King Georges’ Medical University Salmonella Typhimurium Kind gift from Prof. Michael Hensel ATCC, 14028 C57BL6/J (M. musculus) Central Animal Facility, IISc Bangalore, India. .. Recombinant DNA pLJC5-3XMyc-EGFP-PEX26 Addgene 139059 pcDNA3 flag HA: SseI This study Vector backbone from #9021, Addgene pcDNA3 flag HA: ARF1 This study Vector backbone from #9021, Addgene pFPV-mCherry Addgene 20956 pET23A His tagged SseI This study N/A pQE60 myc::sseI This study N/A pQE60 sseI::myc This study N/A SseI, Syt7, and ARF1 (along with mutants) were synthesized commercially GenScript Antibodies Rabbit Anti-LAMP1 Cell Signalling Technology (CST) D401S Anti-FLAG CST 8146 Anti-myc CST 2276 Anti-Pex14 ABclonal A7336 Anti-ESYT1 ABclonal A15410 Anti-ABCD1 Proteintech 60153-1-Ig Anti-pex5 Proteintech 12545-1-AP Anti-ARF1 Proteintech 10790-1-AP Anti-GAPDH Invitrogen T0004 Anti-HA CiteAb 2367 Anti-SYT7 Novus Biologicals NBP2-22420 PIP2 antibody Novus Biologicals NBP2-76433 Goat anti-mouse IgG Alexa fluor 594 CiteAb A-11032 Goat anti-Rabbit IgG Alexa Fluor 488 CiteAb A-11034 Goat anti-rabbit IgG Cy5 Invitrogen A10523 Control Mouse immunoglobulin Invitrogen 500-M00-1MG Control rabbit immunoglobulin PeproTech 500-P00-500μg horseradish peroxidase CST 7074P2 Oligonucleotides and other sequence-based reagents ARF1 Santa Cruz SC-105086 Synaptotagmin VII (SYT7) Santa Cruz SC-41320 E-Syt1 Santa Cruz SC-95714 16 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on D ecem ber 25, 2024 from IP 2402:800:6343:b0f8:400f:1124:9738:8c7d. .. Reagent/Resource Reference or Source Identifier or Catalog Number NPC1 Santa Cruz SC-41588 ABCD1 Santa Cruz SC-41143 On-Target plusTM Control Pool Dharmacon DM D-001810-10-20 RT Primers PEX11B FWD CTTCAGTGCTCAGAGCCAAGCC PEX11B REV GCATGGCCAAGAAGAGAGCAA PEX14 FWD CAGAAAGATGGCGTCCTCGG PEX14 REV TCATCTGTCAGCCCTTTCTTCT PEX19 FWD CCCCCACAGACAGTGAAACC PEX19 REV GTTGAGGCCAGGAGGCATCTC ABCD1 FWD GCCCTCCCTGTGGAAATACC ABCD1 REV AGCTTCTCGAACTTCCAGCC ARF1 FWD GTTTGCTGTGAAGACGGTGTC ARF1 REV AGAGGATCGTGGTCTTCCCT GAPDH FWD TCGGAGTCAACGGATTTGGT GAPDH REV TTCCCGTTCTCAGCCTTGAC ACBD5::FWD HU CTGGAAACGCTGACTGCTTTGC
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